LUEGO: a cost and time saving gel shift procedure
نویسندگان
چکیده
منابع مشابه
LUEGO: a cost and time saving gel shift procedure.
We developed a new approach to prepare DNA probes for electrophoretic mobility gel shift assays that presents a number of advantages compared with the classical approach. The method relies on two complementary oligonucleotides containing the desired transcription factor binding sequence, one of them being extended at its 3' end with a universal tail that complements a third, short oligonucleoti...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2011
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113751